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SCIENCE

THE POWER OF FERMENTATION.
GUTS HEALTH FOR YOUR DOG.

Discover COZY MILK. Natural Postbiotic Wellness.
COZY MILK Bottle

Science of fermentation

History of 10,000 Years+1,700 years
Pillar Function Result
Microorganism The “worker” that performs fermentation Koji mold (Aspergillus oryzae) in COZY MILK
Substrate The “raw material” being transformed Rice in COZY MILK
Environment The “conditions” controlling the process Temperature (55–60°C), Time (6–8 hrs), pH

Fermentation is a metabolic process where microorganisms (bacteria, yeasts, or molds) convert organic compounds—typically carbohydrates like sugars and starches—into simpler compounds, producing energy and creating new substances.

Simple Formula:
Complex Nutrients (hard to digest)
+ Microorganisms (bacteria, yeast, mold)
+ Controlled Environment (temperature, time, oxygen)

Simpler Compounds (easy to absorb)
+ Beneficial Byproducts (vitamins, acids, enzymes)
+ Enhanced Nutrition

Fermentation Revolution

Why fermentation matters
Preservation Extends food shelf life (before refrigeration)
Nutrition Enhances bioavailability of nutrients
Digestion Pre-digests complex molecules
Safety Creates acidic environment that inhibits pathogens
Flavor Develops unique taste profiles

What Happens During Fermentation?

The Three Key Processes

1. Molecules Examples:

• Starch → Oligosaccharides → Glucose

• Protein → Peptides → Amino Acids

• Fats → Fatty Acids + Glycerol

2. METABOLISM (Energy Production)

Microorganisms consume simple sugars

Produce energy (ATP) for growth

Release metabolic byproducts:
• Organic acids (lactic, acetic, citric)
• Gases (CO₂ in beer/bread, minimal in amazake)
• Alcohols (ethanol in wine/beer, trace in amazake)
• Heat

3. BIOSYNTHESIS (Creating New Compounds)

Microorganisms synthesize:
• Vitamins (especially B-complex)
• Enzymes (digestive aids)
• Antimicrobial compounds (bacteriocins)
• Aromatic compounds (flavor, aroma)
• Structural components (cell walls → β-glucan)

The Unique Power of
Koji Enzymes

What Makes Koji Fermentation Unique?
Koji (麹) = Grains (rice, barley, soybeans) inoculated with Aspergillus oryzae mold
The Japanese Innovation: While other cultures fermented with bacteria or yeast, Japan mastered mold fermentation over 1,700 years ago.
Why Koji is Called “The Enzyme Factory”
Aspergillus oryzae produces over 100 different enzymes—far more than bacteria or yeast.

The Three Enzyme Superstars

1. AMYLASE (α and β types)

Function: Starch → SugarsPower
Level: ★★★★★

What it does:

  • α-Amylase: Randomly cuts starch chains → dextrins
  • β-Amylase: Cuts from ends → maltose
  • Glucoamylase: Produces pure glucose

Result in COZY MILK:

  • Natural sweetness (no added sugar)
  • Oligosaccharides (prebiotics)
  • Glucose (instant energy)
2. PROTEASE (acid, neutral, alkaline types)

Function: Protein → Amino Acids
Power Level: ★★★★☆

What it does:

  • Cuts protein chains at specific points
  • Releases all 20 amino acids
  • Creates peptides with biological activity

Result in COZY MILK:

  • Free amino acids (no digestion needed)
  • Umami flavor (glutamic acid)
  • Bioactive peptides
3. SPECIALTY ENZYMES

Phytase: Releases minerals from phytic acid
Lipase: Breaks down fats Cellulase: Digests fiber
Pectinase: Softens plant material

Result in COZY MILK:

  • Enhanced mineral bioavailability
  • Better nutrient absorption
  • Easier digestion

Safety Profile

Why it’s safe (unlike some Aspergillus species):
No Mycotoxins:

  • Wild Aspergillus species produce aflatoxins (toxic)
  • A. oryzae has been domesticated for 1,700+ years
  • Lost ability to produce toxins through selective breeding
  • Recognized as GRAS (Generally Recognized As Safe) by FDA
Non-Pathogenic:

  • Doesn’t infect humans or animals
  • Doesn’t survive in body temperature (37°C)
  • No allergenic properties (when properly processed)
1,700 Years of Safe Use:

  • Consumed daily by millions in Japan
  • Core ingredient in traditional Japanese diet
  • Zero recorded toxicity incidents

The Fukumitsuya Difference 400 Years of Koji Mastery

Traditional Toji (Master Brewer) Techniques:
  • Spore Selection (種麹 – Tane-koji)

    • Proprietary strain maintained for generations
    • Selected for enzyme profile
    • Optimized for amazake production
  • Temperature Control (品温管理)

    • Manual monitoring every 2-3 hours
    • Precise adjustments based on grain appearance
    • “Reading” the koji by sight, smell, touch
  • Mixing Technique (切り返し – Kirikaeshi)

    • Specific hand movements to aerate
    • Redistributes heat evenly
    • Prevents hot spots and uneven growth
  • Water Quality (百年水 – Hyakunen-sui)

    • Ancient underground aquifer water
    • Mineral composition affects koji growth
    • Soft water ideal for enzyme activity
  • Rice Selection

    • Contract-grown premium rice
    • Specific polishing ratio
    • Optimal starch/protein balance

What are Postbiotics?

The Stable Power Behind COZY MILK
Official Definition
Postbiotics are beneficial compounds created during fermentation that remain effective even after the microorganisms are no longer alive.
Heat-Treated Fermentation Product = Inanimate microorganisms + Their beneficial components = POSTBIOTIC

WHY POSTBIOTICS ARE BETTER

Live Probiotics

  • Needs refrigeration
  • Dies during shipping
  • 90% killed by stomach acid
  • Inconsistent results
COZY MILK Postbiotics

  • Room temperature stable
  • Stable during shipping
  • 100% reaches gut
  • Consistent results

How Postbiotics Work

Three Simple Mechanisms:

1. Gut Protection

Organic Acids

Lower pH (6.5 → 5.0)

Harmful bacteria can’t grow Good bacteria thrive

2. Immune Support

β-Glucan

Activate immune cells (macrophages, NK cells)

Better infection defense Balanced immune response

3. Microbiome Enhancement

Oligosaccharides (prebiotics)

Feed good bacteria

Produce MORE beneficial compounds

Long-term gut health

Perfect for Dogs

Why Postbiotics Work Better for Pets:
  • No refrigeration → Easy for pet parents
  • Acid-resistant → Dogs have very acidic stomachs (pH 1-2)
  • Predictable → Same results for every dog
  • Safe → Puppies, seniors, sensitive stomachs
  • Convenient → Pour and serve, no special storage
COZY MILK Bottle

COZY MILK

(Heat-Treated Fermentation)

  • Traditional Fermentation
  • 100% Plant-Based
  • Natural Hydration
  • 9 Essential Amino Acids
  • Postbiotic Power

Postbiotic Compounds:

Organic Acids
  • Lactic acid, Acetic acid, Citric acid
  • Lower gut pH → Inhibit harmful bacteria
  • Enhance mineral absorption (2-3x better)
β-Glucan (from koji mold cell wall)
  • Immune activation
  • Strengthens natural defenses
  • “Trains” immune system
B-Vitamins (produced during fermentation)
  • Energy metabolism
  • Skin & coat health
  • Heat-stable, fully retained

Prebiotic Compounds:

Oligosaccharides (unique to koji)
  • Kojibiose, Nigerose, Maltose
  • Feed beneficial gut bacteria
  • Bacteria produce short-chain fatty acids
    → Anti-inflammatory effects

Science-Backed

LATEST RESEARCH (2024-2025)
Prebiotic-Postbiotic Combination in Senior Dogs
2025 | Nature Scientific Reports

→ 22 dogs, 14 weeks
→ Gut microbiota reshaped
→ Immune function improved
[ Read Study ]
Aspergillus oryzae Fermentation for Dogs
2025 | Journal of Animal Science

→ Enhanced digestibility
→ Improved palatability
→ Better nutrient absorption
[ Read Study ]
Postbiotics: A Novel Approach to Canine Health
2025 | Preprints.org (Meta-Analysis)

→ Meta-analysis of 13 studies
→ TNF-α decreased by 23.97%
→ IL-10 anti-inflammatory increased
[ Read Study ]
Bacillus paracasei Postbiotic Effects in Dogs
2025 | University of Illinois Study

→ Improved nutrient digestibility
→ Enhanced fecal metabolites
→ Positive immune changes
[ Read Study ]
The Influence of ‘Biotics’ on Canine Gut Health
2024 | Veterinary Record

→ Comprehensive review
→ 14 citations
→ Postbiotics promote host health
[ Read Study ]